Experimental infection of sheep with Bovine leukemia virus (BLV): Minimum dose of BLV-FLK cells and cell-free BLV and neutralization activity of natural antibodies
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چکیده
منابع مشابه
Prevalence of bovine leukemia virus (BLV) infection in the northeast of Iran
The purpose of this study was to determine the prevalence of bovine leukemia virus (BLV) in Khorasan Razavi and Khorasan Shomali provinces which are the main provinces located in the northeast of Iran. Total number of 429 blood samples were collected from industrial dairy herds. The samples were categorized based on province, age (2-3, 4-6, and 7-10 years old), calving (≤ 2, 3-5, and > 5) and h...
متن کاملGenetic variation of bovine leukemia virus (BLV) after replication in cell culture and experimental animals.
This article reports the selection of bovine leukemia virus (BLV) variants after continuous passage in cell lines or experimental animals. Two wild BLV strains isolated from 2 naturally infected Holstein dairy cows in Brazil (cow codes: 485 and 141) were used for the experimental infection of 1 sheep and FLK cells, and 1 rabbit and CC81 cells. Viral DNA was isolated several months after infecti...
متن کاملDetermination of Lymphocyte Subsets and Pcna Activity in Sheep Experimentally Infected with Bovine Leukemia Virus (blv)
Lymphocyte phenotypes were determined in sheep experimentally infected with bovine leukemia virus (BLV). BLV infection was detected by serological tests AGID and ELISA. Flow cytometry analysis with the use of specific monoclonal antibodies for CD markers and conjugates labeled with FITC or PE was performed. Proliferating cell nuclear antigen (PCNA) was estimated in lymphocytes of infected and h...
متن کاملComparison of bovine leukemia virus (BLV) and CMV promoter-driven reporter gene expression in BLV-infected and non-infected cells
BACKGROUND: Viral promoters are used in mammalian expression vectors because they generally have strong activity in a wide variety of cells of differing tissues and species. METHODS: The utility of the BLV LTR/promoter (BLVp) for use in mammalian expression vectors was investigated through direct comparison to the CMV promoter (CMVp). Promoter activity was measured using luciferase assays of ce...
متن کاملEstimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
BACKGROUND Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most common neoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistent lymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+ T cells, CD3+ T cells, CD4+ T cells, CD8+ T cells, γ/δ T cells, mono...
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ژورنال
عنوان ژورنال: Revista Argentina de Microbiología
سال: 2019
ISSN: 0325-7541
DOI: 10.1016/j.ram.2019.01.004